Mineral Nutrient Requirements of Chlorella Sorkiniana in Continuous Pure Culture

摘要

Mineral nutrient requirements of Chlorella sorokiniana under suitable sterile conditions were studied in a photothermostat and in two continuous culture units at constant densities of 2 microliters/ml. and 20 microliters/ml. Minimum, optimum, and maximum concentrations for each of the following essential elements are reported: N, P, Mg, S, K, Fe, Ca, Mn, Zn, and Cu. Additional studies with B, Mo, Co, Na, Cl, and ethylenediaminetetraacetate (EDTA) are also reported. Optimum nutrient requirements were compared at growing temperatures of 25 and 39C., and at 4.8% and 0.51% concentrations of carbon dioxide in air. The hydrogen ion requirement was determined. An optimal medium was formulated and evaluated in sterile photothermostatic and pure continuous cultures. The effluent medium was reconstituted after each harvest by replenishing the supernatant medium with essential elements and then was returned to the culture. (Author)