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Method for the quantitative determination of a serum protein in turbid serum and plasma samples, and reagent for using this method.
Method for the quantitative determination of a serum protein in turbid serum and plasma samples, and reagent for using this method.
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机译:定量测定混浊的血清和血浆样品中血清蛋白的方法,以及使用该方法的试剂。
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摘要
The invention relates to a process for the quantitative determination of a serum protein, especially apolipoprotein, in turbid serum or plasma samples by nephelometry, which comprises, after having carried out an immunologic reaction with the corresponding antibody, measuring in the presence of a tenside of the formula CH3-(CH2)m-R-(CH2-CH2-O)n-H (I) in which R is O NH CHCH(CH2)xNH +TR IMAGE Y is H- or CH3-(CH2)q-, m and q, being identical or different each are an integer of from 6 to 26, preferably 6 to 18 n is an integer of from 7 to 50, preferably 7 to 25 and x is 7 or 8 the tenside being used in a concentration of from 10-3 to 10-1% by volume. Alternatively, the measures can be taken after enzymatic degradation of the lipids and subsequent immunologic reaction with the corresponding antibody in the presence of a nonionic tenside of the formula (I), in which R is as defined above, or IMAGE +TR R' being CH3 or C2H5, Further subjects of the invention are the use of a tenside of the cited formula for serum work-up for quantitative determination of an apolipoprotein, and an agent for serum work-up for quantitative determination for an apolipoprotein, consisting of a mixture of at least one lipid-degrading enzyme and a tenside of the cited formula.
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