Method for the quantitative determination of a serum protein in turbid serum and plasma samples, and reagent for using this method

摘要

The invention relates to a method for the quantitative determination of a serum protein, in particular apolipoprotein in cloudy serum or plasma samples by turbidity measurement, in which the measurement is carried out after carrying out an immunological reaction with the corresponding antibody in the presence of a cationic surfactant of the general formula CH3- (CH2) mR- (CH2-CH2-O) nH in which R means IMAGE where Y is H- or CH3- (CH2) q-, m and q, identical or different, integers between 6 and 26, preferably between 6 and 18 , n is an integer between 7 and 50, preferably between 7 and 25 and x 7 or 8, the surfactant in a concentration of 10 - 3 to 10 -? Vol% is used. The measurement can also be carried out after enzymatic degradation of the lipids and subsequent immunological reaction with the corresponding antibody in the presence of a nonionic surfactant of the general formula mentioned in which R has the meanings given above or IMAGE where R 'is CH3 or C2H5, m a integer between 6 and 26, preferably between 6 and 18, n is an integer between 7 and 50, preferably between 7 and 25 and x is 7 or 8. The invention further relates to the use of a surfactant of the general formula mentioned for serum processing for the quantitative determination of an apolipoprotein and an agent for serum processing for the quantitative determination of an apolipoprotein, consisting of a mixture of at least one lipid degradation enzyme and a surfactant of the named general formula.