3-Hydroxymethylceph-3-em-4-carboxylic acids are prepared by esterase-catalysed hydrolysis from the corresponding 3-acyloxymethylceph-3-em-4-carboxylic acids. The esterase used for this purpose is obtained by cultivation of a microorganism from the Basidiomycetes class. This process is used particularly advantageously for compounds of the formula I in which the symbols R1, R2, R3 and B have the meanings stated in Claim 6, in particular for cephalosporin C.
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