METHOD OF URINE ANALYSIS FOR DETERMINING THE PRESENCE OF TUMORS WITH B-GLUCURONIDASE ACTIVITY AND METHOD OF DIAGNOSING TUMOR CELLS WITH B-GLUCURONIDASE ACTIVITY

摘要

THE PRESENT INVENTION RELATES TO A METHOD OF URINE ANALYSIS FOR DETERMINING THE PRESENCE OF TUMORS WITH B-GLUCURONIDASE ACTIVITY. /P P THE METHOD OF DETERMINING THE QUANTITY OF FREE GLUCURONIC ACID IN L 'URINE IS CHARACTERIZED IN THAT: THE URINE IS TREATED WITH A LEAD ACETATE SOLUTION; WE SEPARATE THE PRECIPITY; ADDED DITHIOCARBIZONE TO SURNANTANT LIQUID; WE SEPARATE THE AQUEOUS PHASE; A TETRABORATE IS ADDED TO THE SEPARATE AQUEOUS PHASE AND THEY ARE REACTED WITH M-HYDROXYDIPHENYL TO CREATE A COLORED, WATER-SOLUBLE COMPLEX, THE DENSITY OF COLOR OF WHICH IS DIRECTLY RELATED TO THE AMOUNT OF GLUCURONIC ACID FREE IN THE SAMPLE. /P P APPLICATION TO THE DIAGNOSIS OF TUMOR CELLS WITH B-GLUCURONIDASE ACTIVITY.