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ENZYME CONJUGATES AND THEIR USE IN THE DETERMINATION OF TRI-IODO THYRONINE AND OF ANTI-TRI-IODO THYRONINE ANTIBODY
ENZYME CONJUGATES AND THEIR USE IN THE DETERMINATION OF TRI-IODO THYRONINE AND OF ANTI-TRI-IODO THYRONINE ANTIBODY
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机译:酶结合物及其在测定三碘甲状腺素和抗三碘甲状腺素抗体中的用途
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摘要
A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.
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