Affinity chromatography of Vibrio cholerae enterotoxin- ganglioside polysaccharide and the biological effects of ganglioside- containing soluble polymers

摘要

Columns of polysaccharide, e.g., agarose, derivatives comprising covalently bonded gangliosides quantitatively adsorb the .sup. 125 I- labeled cholera toxin of chromatographed samples. The most preferred derivatives are those wherein the gangliosides are coupled to "macromolecules" native albumin, denatured albumin, poly(L-lysine) and poly(L-lysyl-DL-alanine) graft copolymers! which themselves are covalently bonded to the, e.g., agarose. The soluble ganglioside polymers can prevent the binding of .sup.125 I-labeled toxin to liver membranes as well as block completely the lipolytic activity of cholera toxin on fat cells, and thus are useful in the management of the manifestations of clinical cholera.