REAGENT FOR MEASURING ENZYME ACTIVITY PARTICIPATING IN PURINE METABOLISM

摘要

PURPOSE:To obtain a reagent for measuring purine nucleoside phosphorylase (PNP) activity, adenosine deaminase(ADA) activity, and guanase activity in the blood, in the blood dissolving red corpsucle, and in a culture solution, by combining a substrate solution, coloring solution, enzyme solution, and reaction terminating agent. CONSTITUTION:For PNP, a substrate solution consists of inosine, sodium azide, and 0.05-2mol buffer solution in pH 7-8, for ADA, it consists of adenosine, sodium, azide, and 0.05-0.2mol buffer solution in pH 7-8, and, for guanase, it consists of guanine, sodium azide, and 0.05-0.2mol buffer solution in pH 6-7. An enzyme solution is a solution of 1.5-4mol xanthine oxidase or xanthine oxidase and prine nucleoside phosphorylase in ammonium sulfate suspension or 40-60wt% glycerol solution. A reaction terminating agent consists of 0.1-0.3mol acitic acid solution, etc.