PURIFIED RIBONUCLEASE * OBTAINING METHOD THEREOF AND DISTINGUISHING METHOD NATIVE RNA AND MODIFIED RNA OR HYDROLYSIS OF MODIFIED RNA

摘要

The new enzyme, seminal plasma ribonuclease, has a content of 1 mole of cysteine per mole, a KM of 0.42 mM against poly-r-U, a KM of 0.51 mM against poly-r(A-U) transcript, and is inactive against native RNA. It is obtained either by placing dialyzed seminal plasma on an anion exchanger and then on a cation exchanger and purifying the fraction not adsorbed on the cation exchanger by affinity chromatography, after a gel chromatography step if desired, or by subjecting it directly to affinity chromatography, a support material being used for the affinity chromatography which bears native RNA or a non-hydrolyzable analog of the enzyme substrate.