FOERFARANDE FOER FRAMSTAELLNING OCH RENING AV EN ANTIGEN TILL C-GRUPPEN HOERANDE MENINGOKOCKPOLYSACKARID SOM HAR HOEG MOLEKYLVIKT OCH AER AKTIV MOT AV C-GRUPPENS MENINGOKOCKER ORSAKAD MENINGIT

摘要

A vaccine against Group C meningococcal meningitis consisting of a polysaccharide of which at least 80% by weight has a molecular weight in excess of 1,000,000 daltons is prepared. The polysaccharide was isolated from Group C hexadecyl trimethylammonium bromide cell paste with 1.0M CaCl2 extraction and purified by phenol extraction, ultracentrifugation at 100,000 g. and ethanol fractionation (30%-45% v/v in ethanol).