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VERFAHREN ZUM BINDEN UND TRENNEN FUR DEN KOMPETITIVEN RADIOIMMUNOASSAY UND REAGENZ HIERZU

机译:竞争性放射性免疫分析的结合和分离方法及其试剂

摘要

1. Process for binding, in an aqueous solution, an antigen which is specifically bound to a first antibody, and for separating this bound antigen from antigen which is not bound, for the determination of the antigen concentration in a body liquid with the aid of competitive radioimmunoassay, whereby the first antibody with the specifically bound antigen is separated from the free antigen which is dissolved in the aqueous solution, the separation being carried out by means of a second antibody, which is bound, on the one hand, to a Staphylococcus aureus cell, and, on the other hand, specifically binds the first antibody, and the process comprises adding a reaction accelerator to the aqueous solution, the reaction accelerator accelerating the specific binding of the first antibody by the second antibody, whereby 4-8 percent by weight polyethylene glycol, 5-10 percent by weight dextran, 5-10 percent by weight polyvinylpyrrolidone, 0,5-0,9 molar ammonium sulfate or 0,8-1,2 sodium sulfite are used as reaction accelerator.
机译:1。在水溶液中结合特异性结合第一抗体的抗原,并从未结合的抗原中分离该结合的抗原的方法,以借助于以下方法测定体液中的抗原浓度。竞争性放射免疫分析,其中具有特异性结合抗原的第一抗体与溶解在水溶液中的游离抗原分离,分离是通过第二抗体进行的,该第二抗体一方面与葡萄球菌结合并与另一种抗体特异性结合,该方法包括向水溶液中添加反应促进剂,该反应促进剂促进第一抗体与第二抗体的特异性结合,从而提高4-8%按重量计的聚乙二醇,按重量计5-10%的右旋糖酐,按重量计5-10%的聚乙烯吡咯烷酮,0.5-0.9摩尔硫酸铵或0.8-1.2亚硫酸钠重新用作反应促进剂。

著录项

  • 公开/公告号PT73359A

    专利类型

  • 公开/公告日1981-08-01

    原文格式PDF

  • 申请/专利权人 HOECHST AKTIENGESELLSCHAFT;

    申请/专利号PT19810073359

  • 发明设计人

    申请日1981-07-13

  • 分类号C12Q;C12R;

  • 国家 PT

  • 入库时间 2022-08-22 16:04:21

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