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Method for preserving the electrophoretic properties of lipoproteins and use of the preserved serums
Method for preserving the electrophoretic properties of lipoproteins and use of the preserved serums
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机译:脂蛋白电泳特性的保存方法和保存的血清的用途
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摘要
ei determining the Lipoproteinmusters of lipoproteins, especially LDL, VLDL and HDL lipoproteins in human or animal body fluids such as plasma, plasma fractions, or serum, the difficulty is given that even for short-term storage, the native composition of the lipoproteins is changed irreversibly so that the determination is inaccurate or falsified results. Also control sera are subject to such a change, so that it was not previously possible to preserve the qualitative and quantitative electrophoretic properties of lipoproteins in a control serum.; These difficulties are resolved by the body fluids to be analyzed non-reducing sugar, in particular sucrose, are added, the mixture is lyophilized and the lyophilisate is reconstituted prior to determination. In this way the native electrophoretic properties of lipoproteins are preserved. The conserved in such a manner bodily fluids can also be advantageously used as control sera for plasma or serum testing.
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