A VACCINE FOR COMBATTING PLEUROPNEUMONIA IN PIGS, AND A PROCESS AND A SUBSTRATE FOR THE AEROBIC FERMENTATION OF HAEMOPHILUS PLEUROPNEUMONIAE

摘要

In an improved method of combating against pleuropneumonia pig by inoculation of a vaccine comprising cells of the known microorganism and generally available Haemophilus pleuropneumoniae, parts of such cells, extracts and / or metabolism of the product thereof as active substances, adjuvants, and a buffer, the improvement resulting from the use of a Bordetella pertussis vaccine, a biomass and / or extracts thereof as an adjuvant, concurrently with the new H. pleuropneumoniae vaccine comprising an adjuvant based on B, pertussis. The method and an improved vaccine LEAD protection of pigs against pleuropneumonia attacks without side effects. Moreover is described a new substrate and improves substrate referred to as "CAY" for the cultivation of microorganisms, particularly the bacteria H. pleuropneumoniae, which compared to the known substrates, results in a substantially higher yield of appropriate biomass for the production of H. pleuropneumoniae vaccine, this substrate comprising casaminique acid, yeast extract, glucose and adenine dinucleotide-nicotinamide (NAD) as essential ingredients and it lends itself to the culture of H. pleuropneumoniae in solid medium as well as in liquid medium by small changes in the composition of the substrate. An improved process for the aerobic fermentation of H. pleuropneumoniae in a liquid medium comprising the substrate "CAY", this fermentation being advantageously effected at a temperature of about 37 C, a pH of about 7.1 to 7.4 and has a oxygen concentration in the fermentation medium of about 8 to 12 ppm, and maintaining the oxygen concentration in Desired by varying the stirring speed and / or air flow.