METHOD OF DIAGNOSIS OF DESTRUCTIVE PROCESS IN LUNGS AT ACUTE PHEUMONIA OF CHILDREN

• 1. 通过检测肺部超声检查中的B线诊断肺部状况的设备、系统和方法 [P] . 中国专利： CN110868936A . 2020-03-06
• 2. 用于基于件的过程（批量制造）、特别是注射成型过程的自动的过程监控和过程诊断的方法和实施所述过程的机器或实施所述过程的机器总成 [P] . 中国专利： CN111867806A . 2020-10-30
• 3. METHOD OF DIAGNOSIS OF DESTRUCTIVE PROCESS IN LUNGS DURING ACUTE PNEUMONIA OF CHILDREN [P] . SU1101223A1 . 1984-07-07

  机译： 诊断儿童急性肺炎期间腰椎破坏性过程的方法

• 4. METHOD FOR PREVENTION OF DEVELOPMENT OF VISCERAL AND PARIETAL ADHESIONS AT ACUTE PURULENT-DESTRUCTIVE LUNG DISEASES IN CHILDREN [P] . UA87083U . 2014-01-27

  机译： 预防儿童急性脓性破坏性肺疾病内脏和胎腔黏附发展的方法

• 5. METHOD OF DIFFERENTIAL DIAGNOSIS OF POLYSEGMENTAL PLEUROPNEUMONIA AND ACUTE PURULENT DESTRUCTION OF THE LUNGS OF CHILDREN [P] . SU1500943A1 . 1989-08-15

  机译： 多发性肺炎的鉴别诊断方法和儿童腰椎急性脓性破坏

• 1. Use of various CT imaging methods for diagnosis of acute ischemic cerebrovascular disease [J] . Gang Wang, Xue Cheng, Xianglin Zhang 中国神经再生研究（英文版） . 2013,第007期
• 2. Role of Ultrasound Lung Comets in the Diagnosis of Acute Heart Failure in Emergency Department:rA Systematic Review and Meta-analysis [J] . LIAN Rui, ZHANG Guo Chao, YAN Sheng Tao, 生物医学与环境科学（英文版） . 2018,第008期
• 3. Clinical Diagnosis and Treatment Characteristics of Acute Respiratory Infections in Children and New Developments in Laboratory Testing [J] . Yueliao Ma, Lingyu Lu, Qiangcai Mai 儿科学期刊（英文） . 2021,第001期
• 4. Rule-based Medical Diagnosis With Learning: Application To The Diagnosis Of Acute Renal Failure In Children [C] . Kurzynski, M.W., Sas, . 2001
• 5. Characterizing Pulmonary Nodules using Machine and Deep Learning Methods to Improve Lung Cancer Diagnosis [D] . Shen, Shiwen. 2018
• 6. Clinical and Laboratory Methods in Diagnosis of Acute Appendicitis in Children [O] . Mojca Groselj-Grenc, Stane Repše, Dubravka Vidmar, 2007
• 7. Features antibiotic therapy in children with acute destructive lung disease [O] . Дейнека К.М. 2017

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. New Progress in Clinical Diagnosis, Treatment and Laboratory Testing of Acute Respiratory Infections in Children [J] . Huan Zhao ,Xiaoyu Jiang ,Lingyu Lu . 美国分子生物学期刊(英文) . 2022,第1期
• 3. 肺部疾病的病理诊断北京儿童医院病理科 [C] . 周春菊 . 第5届全国儿童支气管镜医学协作组会议 . 2012
• 4. G、GM试验对非粒细胞缺乏儿童侵袭性肺部真菌感染的诊断意义 [A] . 张晶 . 2018