Turbidity test for endotoxins - useful for quantitative tests with e.g. biological fluids, drugs and parental compsns

摘要

Endotoxin content of an aq. liquid is determined by mixing the liquid with aq. lysate of washed amoebocyte cells from the blood of the horseshoe crab (the lysate is obtd. by water lysing washed amoebocyte cells and is buffered at pH 6.5-6.8). The lysate is of such concn. that u ml. is reactive with 0.1 ml. endotoxin contg. 1 ng/ml to develop, after 60 mins. at 37 degrees C, turbidity that imparts an absorbency between 0.07-0.2 at 360 nm. The mixture is incubated and the absorbence is determined. Qualitative and quantitative test suitable for screening drugs, biological fluids, water samples, parenteral prepns., medical equipment etc. for pyrogens by a simple, effective and reliable method, even with low levels of endotoxins.