PRODUCTION OF RED DYE CHRYSANTHEMIN BY TISSUAL CULTURE OF EUPHORBIA MILLII CH. DES MOULINS

摘要

PURPOSE:To collect a red dye chrysanthemin, by cultivating a callus derived from a plant tissue of Euphorbia Millii Ch. des Moulins in a culture medium containing 2,4-diclorophenoxyacetic acid, sucrose, etc. under the irradiation with light at a specific lux value or more. CONSTITUTION:Leaves of Euphorbia Millii Ch. des Moulins which is a plant of the genus Euphorbia are sterilized and cut to small pieces. The pieces are cultivated to form a callus. The resultant callus is then cultivated in a culture medium containing 2,4-dichlorophenoxyacetic acid in a concentration of 10-4-10-7M, sucrose in an amount of 1-10wt%, organic substances in a very small amount, various natural extracted substances, etc. in the well-known inorganic synthetic agar culture medium. The cultivation is carried out under irradiation with light at 100 luxes or more. A red dye chrysanthemin of the formula is separated, collected and purified by the solvent extraction, various chromatographic methods, etc.