A procedure for the preparation of a Live virus vaccine of Feline infectious peritonitis

摘要

Procedure for preparing a Live virus vaccine of Infectious peritonitis Cats (PIF). To isolate the virus from the BIP of organs or tissues of infected animals with the same, disaggregating the organs or tissues in isolated cells or used as Organ cultures.The virus replicates in cells obtained directly from tissues of CAT and Feline obtained originally Continuous cell lines. The virus replicates at a temperature comprendidad between approximately 30 and 40 c in cells that develop in a variety of Culture Media.After checking that the virus has multiplied in the normal Cell Cultures, Cell Cultures can be Frozen and thawed once or more times, and the virus in the overlying Fluid pass to other crops of normal cells may be so seriad Or Stable cell lines exhibit at least 80 times without change.Increasing the content of virus Fluid with each serial Passage Cell, reaching a maximum concentration. Vaccines were prepared by diluting the FIP virus suspensions, and also with other viruses known as polyvalent Vaccines of felines.