Apparatus and method for antibody screening, typing and compatibility testing of red blood cells

摘要

Apparatus and method for antibody screening, typing and compatibility testing of red blood cells a dual cavity (22, 32) substrate having a sample compartment (24) for selectively discharging the fluid under test is rotatably mounted in a holder. A monolayer of red blood cells is centrifugally developed on the cavity (22, 32) surfaces of the substrate (10) and, after typing, is subjected to the presence of an antibody which antibody may or may not have an affinity for the antigen of the first layer of red blood cells. A second monolayer of red blood cells is layered on the antibody. One of the two layers of blood cells or the antibody is the element being tested. Should agglutination of the second layer of red blood cells occur, the test is positive. Equipments for various substrate surface preparation procedures as well as for washing, lysing and optical inspection procedures are also described. A method for conducting various tests perform- able by the apparatus is disclosed.