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Enzyme immunoassay for determining antigen specific antibodies and test kit for carrying out this assay
Enzyme immunoassay for determining antigen specific antibodies and test kit for carrying out this assay
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机译:用于确定抗原特异性抗体的酶免疫测定法和用于进行该测定法的测试试剂盒
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摘要
A highly sensitive enzyme immunoassay procedure is disclosed for determining IgM-, IgA-, IgG- and IgE- antibodies which are specific to antigens by coating a solid support with an antibody which selectively binds the antibody to be determined in a serum specimen and reacting the selectively bound antibody with an enzyme labeled microbe or antigen and then determining the enzyme activity of the reaction product as a measure of the antibody to be detected. As the antigen can be effectively labeled a relatively impure antigen can be labeled and used in high dilutions resulting in a very sensitive assay; purification by column chromatography is unnecessary. A kit for conducting the assay is also disclosed.
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