METHOD AND APPARATUS FOR DETERMINATION OF GLUTAMIC ACID OXALOACETIC ACID TRANSAMINASE AND GLUTAMIC ACID PYRUVIC ACID TRANSAMINASE

摘要

PURPOSE:To determine the content of the titled enzyme necessary for the diagnosis of diseases, in high accuracy, by adding alpha-ketoglutaric acid to the specimen solution, adding L-aspartic acid or L-alanine to the mixture to cause the enzymatic reaction, and determining the produced carbonate. CONSTITUTION:A specimen solution containing glutamic acid oxaloacetic acid transaminase (abbreviated to GOT) and glutamic acid pyruvic acid transaminase (abbreviated to GPT) is added with alpha-ketoglutaric acid. L-aspartic acid or L- alanine is added to the mixture to effect the enzymatic reaction of formulaI or formula II. The produced L-glutamic acid is made to react with glutamic acid decarboxylase to effect the enzymatic reaction of formula III, and the produced carbon dioxide gas is absorbed through a membrane to an absorbent. GOT and GPT and can be determined from the variation of the electrical conductivity of the absorbent liquid.