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Process for the production of a culture of cells of vertebrates transform such a culture use of antigeno hepatitis B surface product of said process vector expression and particle DNAIncluding such antigeno
Process for the production of a culture of cells of vertebrates transform such a culture use of antigeno hepatitis B surface product of said process vector expression and particle DNAIncluding such antigeno
A vertebrate cell culture is enabled to produce a desired polypeptide by transformation with a vector whose DNA comprises (a) means for replication in the host; (b) a promoter; and (c) a gene encoding the polypeptide, under the control (b). E.g. a recombinant genome was constructed comprising DNA from (a) the SV40 virus with excision of the VP-1 gene (-hence it is noninfective); (b) the HBsAg gene; and (c) pBR322 (rendering it TETR, and allowing cloning in E. coli). Cells of the CV-1 line of monkey kidney fibroblasts were co-infected therewith and incubated. Lysis occurred, liberating HBsAg which is required for hepatitis vaccine. Non-lytic vectors for expressing HBsAg were also prepared.
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