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Procedure for selection of cell clones to submerged cultures.
Procedure for selection of cell clones to submerged cultures.
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机译:选择细胞克隆至深层培养的程序。
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摘要
In this process, a range of individual devices which are known per se are employed jointly to increase the number of cell clones to be tested per unit of time, in which process a) sterile microtitre plates are charged automatically with nutrient solutions under sterile conditions; b) each well of the microtitre plate is automatically inoculated with one cell; c) the inoculated microtitre plates are stacked and, in a shaker incubator incubated under submerged-culture conditions; d) a filtrate of the nutrient solution or an extract of the cell culture is prepared, and, in this filtrate or extract, the amount of the desired metabolic product is measured by a method known per se.
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