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method and reagent for the determination of potassium in a human or animal serum or plasma

机译:测定人或动物血清或血浆中钾的方法和试剂

摘要

Determn. of the K concn. in human or animal serum or plasma is effected by adding the sample to an alkaline medium contg. NaB(Ph)4 and measuring the turbidity resulting from the formation of KB(Ph)4, with the improvement that the alkaline medium also contains a solubilising agent (I) for serum or plasma proteins. (I) is pref. a buffer which also provides the required alkalinity, esp. tris(hydroxymethyl)aminomethane ("Tris"). The medium pref. also contains a solubilising agent for lipids, esp. a K-free soap of a higher fatty acid. Na dilaurate can serve as solubilising agent for both lipids and proteins. The process eliminates the steps of protein pptn. and centrifugation.
机译:确定。 K concn。通过将样品加入到连续的碱性介质中,可以使人或动物体内的血清或血浆水平升高。 NaB(Ph)4并测量由KB(Ph)4形成而产生的浊度,改进之处在于碱性介质还包含用于血清或血浆蛋白的增溶剂(I)。 (I)是首选。还可提供所需碱度的缓冲液,特别是三(羟甲基)氨基甲烷(“ Tris”)。中等偏爱。还含有脂类的增溶剂,特别是。一种不含钾的高级脂肪酸皂。二月桂酸钠可以用作脂质和蛋白质的增溶剂。该过程消除了蛋白pptn的步骤。和离心。

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