procedure for the determination of human hla - d (r) and gensonde tissue-typing reagents for use in the procedure

摘要

A new method for determination of polymorphism of human MHC alleles for tissue typing, preferably of HLA-D tissue types. The method is characterized in that a probe, which hybridises to a base sequence in the MHC, is used in restriction fragment analyses of DNA from different individuals, and that the size distribution of DNA fragments, hybridising to the probe and originating from one first individual, is compared with the corresponding fragment distribution from a second individual or with the corresponding distribution reflecting a particular type of polymorphism. The comparisons are carried out in order to determine: a) the genetic matching of said other individual for thepurpose of transplantation or blood transfusion, b) the relative risk of the first individual to acquire a particular disease related to one or more of the alleles of the MHC, c) the biological paternity or maternity of the first individual or d) if the first and second individual exhibit the same polymorphism of one or more particular MHC allele, i.e. for use in forensic medicine.