procedure for the determination of human hla - d (r) and gensonde tissue-typing reagents for use in the procedure

摘要

One completely new method of polymorphous measurement to human MHC allele for tissue typing, preferably HLA-D tissue types. The method is characterized in that a probe, its to a base sequence MHC hybridises, restricted fragment from different individuals for DNA is analyzed, the distribution of particles of that DNA fragmentation, hybridising to probe and from one first individual, from the second individual or in the case where corresponding distribution reflects a kind of polymorphous specific type compared with corresponding fragment distribution. Compare that do be for determination: a) matchings of the heredity of other individuals of the thepurpose for transplanting or transfusing blood, b) relative risk of the first individual obtains a specific disease related with the one or more allele of MHC, c) patriarchy or maternal instinct or d) of the biology of the first individual, although first and second identical polymorphic of individual showpiece of one or more specific MHC allele, i.e.For medical jurisprudence.