PROCESS FOR THE DETERMINATION OF GLUTAMATE-OXALATE-ACETATE TRANSAMINASE OR GLUTAMATE-PYRUVATE TRANSAMINASE

摘要

In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of alpha -ketoglutarate in a buffered solution, the alpha -ketoglutarate formed is reacted with gamma -aminobutyrate in the presence of gamma -aminobutyrate transaminase with formation of succinate semialdehyde, NADP is reduced with the latter in the presence of succinate semialdehyde dehydrogenase to give NADPH, and the latter is measured either directly or after conversion with a tetrazolium salt and an electron carrier such as diaphorase, phenantroline methosulphate or phenazine methosulphate to a formazane dye. A reagent suited for use with this method contains gamma -aminobutyrate transaminase, succinate semialdehyde dehydrogenase, gamma -aminobutyrate, glutamate and buffer, as well as either oxalacetate or pyruvate, and optionally a tetrazolium salt, an electron carrier and a surface-active agent.