Bovine mastitis vaccine, process for preparing it, and method for detecting efficacy thereof

摘要

A vaccine for the prevention and/or control of gram-positive cocci, e.g. Str. agalactiae and S. aureus, induced bovine mastitis infections comprises at least one of(a) at least one encapsulated S. aureus antigen which is derived from a bovine isolated and inactivated S. aureus strain; and(b) at least one unencapsulated S. aureus antigen which is derived from a bovine isolated and inactivated S. aureus strain; together with(c) at least one of Str. agalactiae antigen derived from a bovine isolated inactivated Str. agalactiae; the antigen being derived from at least one inactivated Str. agalactiae, Type I (la or Ib or Ic or mixtures thereof), Type II and Type III;optionally containing an adjuvant; andsuspended or dissolved in a pharmaceutically acceptable vehicle.;The invention includes an enzyme linked immunosorbent assay (ELISA), for assaying the humoral immune response of the lactating bovine mammary gland to detect antibody variables associated with the vaccinated or suspended infected bovine.