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Heat shock process for the isolation of bacterial protein
Heat shock process for the isolation of bacterial protein
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机译:热激过程分离细菌蛋白
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摘要
There is disclosed an improved process for the isolation of K- 99 outer membrane protein pili from Escherichia coli cells. The improved process involves a heat shock treatment of the E. coli cells in a buffered solution to afford a release of the K-99 pili into solution which on standing results in the aggregation and precipitation of the pili. The thus isolated K-99 protein pili is useful in the preparation of vaccines.
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