PRODUCTION OF ASPERGILLUS BY PROTOPLAST FUSION

摘要

PURPOSE:To obtain an aspergillus strain having high activity of produce protease and glutaminase, by preparing a protoplast fusion cell of a strain having high activity to produce protease and belonging to Aspergillus genus and a strain having high activity to produce glutaminase, and culturing the obtained fusion cell in a hypertonic regeneration medium. CONSTITUTION:A protoplast is obtained from a strain having high activity to produce protease and belonging to Aspergillus genus (e.g. Aspergillus sojae ATCC 42249) and a strain having high activity to produce glutaminase [e.g. Aspergillus sojae 262 (FERM-P No.2128)] by the use of a cell wall lyase (e.g. a combination of beta-1,3-glucanase and chitinase). The fused cell obtained by the protoplast fusion is cultured in a hypertonic regeenration medium (e.g. a medium obtained by adding agar to the soup of malted rice containing sorbitol), and an aspergillus strain capable of highly producing both protease and glutaminase is separated from the cultured product.