A process for the preparation of obligate methylotrophic-bacteria, which express contained foreign DNA, which comprises(a) isolating a plasmid from an obligate methylotrophic bacterium,(b) preparing from this and from a plasmid with selection markers a hybrid plasmid with a replicon inherent to the obligate methylotrophic bacterium.(c) introducing this hybrid plasmid by transformation into a host organism and amplifying it there.(d) after selection, treating the clones with a suitable conjugative plasmid and abolishing the mobilizability defect,(e) conjugating the clones thus obtained with plasmid-free obligate nethylotrophic bacteria as the recipient and(f) selecting the desired clones.
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