Plasmid cloning and expression vectors for a protein in a microorganism, comprising at least the expression promotor of beta-glucosidase in the yeasts; these vectors containing microorganisms; fermentation process and enzymes so obtained

摘要

The present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism, which comprises at least one structural gene which codes the synthesis of the said protein and elements which ensure the expression of the said structural gene in a microorganism, and wherein the promotion of the structural gene is ensured by the expression promotor of the beta -glucosidase gene in yeasts. The present invention also relates to microorganisms transformed by the said vectors, in particular a transformed strain of S. cerevisiae, a fermentation process using the said vectors, and the enzymes prepared by the said process, in particular beta -glucosidase.