Method for preparing and analyzing single branched antibody

摘要

The invention relates to a method of a preparation of monoclonal antibody derived from a crosslinked fibrin derivative including the steps of: (i) obtaining a crosslinked fibrin derivative or extract containing same; and (ii) forming an antibody to said derivative or extract by cloning antibody producing cells from an animal having been administered thereto said derivative or extract. A screening assay for use in the abovementioned method which includes the steps of: (A) coating a surface with an antigen selected from a (i) crosslinked fibrin derivative; (ii) extract containing said derivative; or (iii) fibrinogen degradation product. (B) contacting said antigen with a monoclonal antibody derived from crosslinked fibrin derivative; and (C) subjecting the complex formed in step (A) to a signal amplification step and an assay procedure for detection of the presence of a crosslinked fibrin derivative in an animal body fluid including the steps of: (i) contacting a monoclonal antibody prepared from a crosslinked fibrin derivative with a fluid sample suspected of containing an antigen derived from a crosslinked fibrin derivative or comprising a crosslinked fibrin derivative per se; and (ii) subjecting the complex formed in step (i) to a signal amplification step are also provided.