REAGENT FOR ASSAYING HYDROGEN PEROXIDE AND METHOD OF QUANTITATIVE ASSAY FOR HYDROGEN PEROXIDE

摘要

A reagent suitable for assaying a very small amount of hydrogen peroxide is composed of a moiety having a residue formed by removing one hydrogen from an active methylene group or an active methine group and a fluorescing moiety. Hydrogen peroxide contained in a sample liquid is determined by reacting the sample with the foregoing reagent, a hydrogen donor, and peroxidase and measuring the intensity of the fluorescence emitted from the fluorescing material formed by the reaction.