METHOD FOR THE PREPARATION OF ANTIGENS OF THE VIRAL HEPATITIS NANB, AND APPLICATION TO THE PREPARATION OF A REACTION AGENT ALLOWING THE DIAGNOSIS AND PROGNOSIS OF INFECTIONS CAUSED BY THE VIRUSES OF VIRAL HEPATITIS

摘要

Method for the preparation of purified antigens of viral hepatitis NANB and application. This method is particularly characterized by the selection of serums or liver extracts wherein the presence of NANB virus has been recognized, the ultracentrifugation to concentrate said viruses in the centrifugation base, the collection and treatment of said base by means of a non ionic detergent that is allowed to incubate during 1 to 24 hours at a temperature comprised between 0 and 37?oC, a fractionning to isolate the fractions containing purified the Ag NANBc, and then, if desired, said fractions are subjected to the action of an ionic detergent, incubation during 1 to 24 hours approximately at a temperature comprised between 0 and 37?oC, and the Ag NANBe is isolated. Application to the obtention of a reaction agent allowing particularly the diagnostic of viral hepatitis NANB.