METHOD AND REAGENT FOR QUANTITATIVE DETERMINATION OF ANTI-STREPTOLYSIN O ANTIBODY

摘要

PURPOSE:To improve the accuracy of quantitative determination by mixing serum and the latex of an insoluble carrier which is sensitized with streptolysin O and has 0.2mum grain size in such a manner that the final concn. attains 0.05wt%, inducing a latex agglutination reaction by the antigen-antibody reaction and measuring light absorbency at 530-600nm wavelength. CONSTITUTION:The serum and the latex of the insoluble carrier which is sensitized with the streptolysin and has about 0.05-0.2mum grain size are mixed with a phosphoric acid buffer soln., etc. as a medium in such a manner that the final concn. of the insoluble carrier attains 0.05wt% to prepare a mixed liquid. The mixed liquid induces the latex agglutination reaction by the antigen- antibody reaction and the light absorbency of the reaction liquid after the reaction is measured by using the adequate wavelength of 530-600nm. The optical path length of the cell in the absorbency measurement is preferably about 5-10mm.