DETERMINATION OF SUBSTRATE BY BILIRUBIN OXIDASE

摘要

PURPOSE:To carry out the determination of a substrate in high workability, simplicity and accuracy, without using an unstable reagent, by reacting a substrate such as phenol, aniline, etc. with bilirubin oxidase in the presence of a coupler, and determined the produced dye. CONSTITUTION:A substrate composed of a phenolic compound or an aniline compound of formula (X is hydroxyl or amino; Z is substituent group other than X, e.g. H, halogen, lower alkyl, lower alkoxy, amino, hydroxyl, carboxyl, sulfonic acid group, etc.; n is 1-5; plural Z may be same or different and may form a ring at the o- and m-positions of X) is made to react with bilirubin oxidase in the presence of a coupler. The substrate of formula can be determined by determining the produced dye.