NOVEL BACILLUS SUBTILIS CONTAINING THERMOSTABLE BETA-GALACTOSIDASE AND PRODUCTION OF THERMOSTABLE BETA-GALACTOSIDASE

摘要

PURPOSE:To product beta-galactosidase having improved thermostability industrially advantageously, by transducing DNA obtained by integrating a DNA fragment carrying specific genetic information into vector DNA for Bacillus subtilis into Bacillus subtilis, and cultivating the Bacillus subtilis. CONSTITUTION:Recombinant DNA obtained by integrating DNA fraction carry ing genetic information of thermostable beta-galactosidase collected from Bacillus stearothermophilus into a vector for Bacillus subtilis is transduced into Bacillus subtilis, which is cultivated. Then, thermostable beta-galactosidase accumulated in the culture material is collected. The integration of the chromosome DNA into vector DNA can be carried out by scissoring the chromosome DNA and the vector DNA with a restricted enzyme to prepare a chromosome DNA frag ment and a vector DNA fragment, and treating a mixture of them with DNA ligase. DNA ligase derived from phage T4 is preferably used as the DNA ligase.