CYCLIC DOUBLE-STRANDED DNA, PROCESS FOR ITS PREPARATION, MICROORGANISM CONTAINING SAID CYCLIC DOUBLE-STRANDED DNA, AND PROCESS FOR PREPARING PROTEIN CONTAINING ANTIGEN DETERMINANT SITE OF POLIOVIRUS CAPSIDE PROTEIN VP1 USING THE SAME

摘要

A process for preparing a protein having an antigen determinant site of capside protein VP1 utilizing cyclic double-stranded DNA containing high expression promotor/operator, SD sequence under its control, and a DNA portion which codes an antigen determinant site of capside protein VP1 of attenuated poliovirus strain. This invention enables to produce the protein in a large amount as a blended or non-blended protein.