Enzyme-linked immunoassay, assay kits and pair-binding compounds

摘要

An immunoassay, for measuring the amount of a ligand present in a medium, uses a luciferase-labelled binding pair member selected from a ligand and a receptor, in which the luciferase is linked to the binding pair member via its reactive sulfhydryl group and is thereby reversibly inactivated.;A kit, for use in an immunoassay to determine the presence of a ligand in a medium, which comprises a plurality of containers, a first container containing luciferase-labelled receptor or ligand (a purified version of the ligand to be assayed) and a second container containing receptor which may be immobilised.;New compounds have a reactive group which can react to cause reversible inactivation of luciferase and a group which can react with one or more sites on a binding receptor or ligand.