TRYPTOPHAN OPERON, PEPTIDE AND PROTEIN CODED THEREBY, UTILIZATION OF TRYPTOPHAN OPERON GENE EXPRESSION AND PRODUCTION OF TRYPTOPHAN

摘要

PURPOSE:To efficiently produce the titled tryptophan in a large amount, by cultivating a microorganism transformed by a DNA sequence capable of coding an enzymic group of a tryptophan synthetic system, etc., in a culture medium. CONSTITUTION:A chromosomic gene obtained by extracting microbial cells of Brevibacterium lactofermentum, etc., of the genus Brevibacterium is cleaved with a restriction enzyme to give a DNA, consisting of DNA expressed by the formula containing an operator region controlling the synthesis of m-RNA, promoter region controlling the synthesis of m-RAN, etc. The resultant DNA is then subjected to ligation reaction with a plasmid vector to afford a recombinant DNA, which is further introduced into a variant strain requiring tryptophan to carry out transformation and provide coryneform bacteria having the ability to produce tryptophan. The resultant bacteria are then aerobically cultivated in a culture medium containing a carbon source, nitrogen source, etc., to produce and accumulate the aimed tryptophan in the culture medium.