MASS-PRODUCTION OF INSECTICIDAL PROTEIN BY EXPRESSION OF INSECTICIDAL PROTEIN GENE IN ESCHERICHIA COLI

摘要

PURPOSE:To mass-produce an insecticidal protein, by forming an expression plasmid, containing an insecticidal protein of Bacillus thuringiensis aizawai IPL strain and capable of expressing the strain in microbial cells. CONSTITUTION:An expression vector, e.g., expression vector pUC18 holding Lac promoter, expression vector pKK223-4 holding Tac promoter which is a powerful promoter for Escherichia coli and terminator for rrbB ribosome RNA, etc., is linked to an insecticidal protein gene of Bacillus thuringiensis aizawai IPL strain to form an expression vector holding the insecticidal protein gene. The resultant expression vector is then introduced into a host microorganism, e.g. Escherichia coli JM103 strain, etc., to give a transformant microorganism, which is then cultivated in a culture medium to mass-produce the aimed insecticidal protein.