METHOD OF TISSUE CULTURE FOR DUBOISIA LEICHHARDTU F. MUELL

摘要

PURPOSE:In producing a propane alkaloid useful as an agalgesic, etc., by subjecting a plant of the genus Buboisia to tissue culture, to improve yield, productivity, etc., by using a medium having a high ratio of NH4 ion to nitrate ion. CONSTITUTION:A plant such as Duboisia myoporoides R. Br, etc., belonging to the genus Duboisia, is subjected to tissue culture by using a medium comprising an inorganic component (e.g., nitrogen, phosphorus or potassium) and a carbon source (e.g., sucrose or ethanol) as essential components blended with a plant hormone, a vitamin and amino acid. In the operation, a ratio of ammonium ion/nitrate ion in the medium is adjusted to =0.2 to carry out the tissue culture. Consequently, a tissue culture mixture containing a large amount of tropane alkaloid such as scopolamine, hyoscyamine, etc., is obtained and extracted with a proper solvent to advantageously give the tropane alkaloid.