Method for obtaining and glycosaminoglycans fractionation of an extract of animal tissues Enzymatic digestion

摘要

The process consists of (a) passing enzyme digestion extract from animal tissues, consisting of lungs, trachea and cartilage from cattle, pigs and sheep, through separate selective ultra-filtration membranes to remove nucleic acids, high mol. wt. polymers (peptides, amino acids and muco-polysaccharides) and salts, and obtain glycosamino-glycans in liquid phase; (b) treating the soln. with quat. ammonium cations in butanol phase to form complexes; (c) purifying the complex soln.; and (d) treating the soln. with NaCl to isolate the glycomamino-glycans in solid phase. The ultra-filtration membranes are of mol. wt. between 1000,000 D and 3000 D.