Procedure for the determination of the binding capacity of Thyroxine binding globulin

摘要

Procedure to determine the binding capacity of the g / lobulina Thyroxine Binding.Comprising: (a) to investigate the Serum Sample incubated with T3 or T4 labeled with peroxidase Enzymes known as B - Galactosidase or United through a Spacer with a length of 3 to 8 Carbon Atoms such as Aminobutyric acid or Glycine; (b) Co incubated with an antibody Ntra T4 or TGB assets against the FC portion of the Antibody.In less than 5 minutes in the case of anti T4; (c) separating the phases; and (d) to measure the Enzyme marking one of the phases. Applicable in Medicine, where the determination of the binding capacity of TGB has special significance in the diagnosis of the Thyroid Gland.