Method to determine the binding capacity of thyroxine-binding globulin

摘要

1. Process for the determination of the binding capacity of thyroxine-binding globulin (TBG) according to the principle of heterogeneous enzyme immune assay, characterised in that one incubates the serum sample to be investigated with an enzyme-labelled T4 or T3 , in which T4 or T3 and enzyme are bound via a spacer, and immobilised antibody against TBG or T4 or dissolved antibody against TBG or T4 and immobilised antibody against the Fc part of the antibody against TBG or T4 , separates the phases and measures the labelling enzyme in one of the phases.