HYBRID CELL LINE FOR PRODUCING COMPLEMENT-FIXING MONOCLONAL ANTIBODY TO MOUSE I-A ANTIGEN AND TO I-A ANALOGOUS ANTIGENS ON HUMAN AND OTHER ANIMAL LYMPHOCYTES, ANTIBODY AND METHODS

摘要

Hybrid cell line for production of monoclonal antibody to mouse I-A antigen and to I-A analogous antigen on human and other animal lymphocytes is described. The hybrid is formed by fusing P3X63-Ag8-653 myeloma cells with spleen cells from A.TH (H-2t2) mouse immunized with A.TL (H-2t1) mouse lymphocytes. The monoclonal antibody reacts with I-A analogous antigens in a wide range of animal species.