A METHOD OF DETECTING OR DETERMINING HISTAMINE IN HISTAMINE CONTAINING MATERIALS, PARTICULARLY BODY FLUIDS AND AN ANALYTICAL MEANS FOR USE IN SUCH METHOD

摘要

Histamine is detected or determined selectively in a sample by causing the sample to contact glass microfibers in such a quantitative proportion as will permit the histamine present to bind to the fibers. The amount of bound histamine may be determined by competitive determination in the presence of labelled histamine on the basis of a standard curve, or may be determined directly by conventional coupling reactions. The method is simple and particularly useful for allergy diagnostics because it exhibits good correlation with known fluorometric methods.