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PROCESS FOR IDENTIFYING COMPOUNDS WHICH CAN ABSORB OR RELEASE ELECTIONS AFTER CATALYSIS BY ELECTIONS

机译:在通过选举催化后可以吸收或释放选举的化合物的过程

摘要

The process uses HPLC and electrochemical identification methods, and is characterized by the insertion of a sample containing one or several compounds to be determined on an HPLC column, the sample being eluted with a suitable elution means. The eluate is then passed through a mini-column filled with a carrier substance to which immobilized oxidases and possibly other enzymes are linked, the pH of the eluate not being modified, after which the enzyme-oxidized or -reduced compound in the solution which emerges from the mini-column is quantitatively determined by electrochemical detection.
机译:该方法使用HPLC和电化学鉴定方法,其特征在于在HPLC柱上插入含有一种或几种待测定化合物的样品,该样品用合适的洗脱方法洗脱。然后使洗脱液通过填充有载体物质的微型柱,固定化氧化酶和可能的其他酶与载体物质连接,洗脱液的pH值未改变,此后溶液中出现酶氧化或还原的化合物通过电化学检测来定量测定来自小柱的值。

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