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Kit for the enzymatic labelling of nucleoside triphosphates, and process for the preparation of nucleoside triphosphates labelled with 32P or 35S with 32PO4H3 or 35S-PO3Na3
Kit for the enzymatic labelling of nucleoside triphosphates, and process for the preparation of nucleoside triphosphates labelled with 32P or 35S with 32PO4H3 or 35S-PO3Na3
The invention relates to kits for the preparation of nucleoside triphosphates (NTP, dNTP) or their derivatives by means of enzymatic reagents which are not nucleoside-specific, and to simple processes employing these kits for labelling with the isotopes 32P or 35S by means of H332PO4 or Na3PO335S without discrimination at the alpha ,ss- or the gamma -position of the nucleotides.
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机译:本发明涉及用于通过非核苷特异性的酶试剂制备三磷酸核苷(NTP,dNTP)或其衍生物的试剂盒,以及涉及使用这些试剂盒标记同位素2 P的简单方法借助于H 3 3 2 PO 4或Na 3 PO 3 3 5 S或3 5 S,而没有区分核苷酸的α,ss或γ位。
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