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LECTIN-ANTIBODY SANDWICH ASSAY FOR DESIALYLATED GLYCOPROTEINS
LECTIN-ANTIBODY SANDWICH ASSAY FOR DESIALYLATED GLYCOPROTEINS
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机译:莱辛抗体夹心法检测去糖化糖蛋白
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摘要
A method for determining the presence of desialylated glycoproteins soluble in biological fluids. The method comprises bringing a sample of biological fluid into contact with a sufficient amount of a suitable lectin capable of binding selectively on the desialylated glycoprotein to produce a complex. The resulting complex is extracted separately from the biological fluid. The extracted complex is contacted, under suitable conditions, with at least one detectable antibody to an antigenic determinant on the desialylated glycoprotein and capable of binding selectively on the glycoprotein present in the complex. The presence of antibody so bound is detected, thereby determining the presence of desialylated glycoproteins in the biological fluid.
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